Novel and human-orientated running interface for smooth operation. The adopted fluorescent real-time detection mode realizes simultaneous amplification and detection in the same tube without need of post experimental treatment. Advanced thermoelectric technology ensures fast and steady heating and cooling of the ultra-fast heat cycling system. Multi-point temperature control ensures consistent temperature of 96 sample wells. It can create a temperature gradient with 4 Thermo-electric modules. Stable and accurate 1～36C gradient function makes optimising PCR conditions simple and easy.